Pause

Rederivation Core

 

 REDERIVATION:

 

   What is Rederivation?

The procedure by which an early, pre-implantation stage embryo is isolated from the reproductive tract of one mouse (donor female) and transferred to the reproductive tract of a mouse (recipient female) made pseudopregnant in order to allow implantation, gestation, and birth.   The rederivation procedure consists of collection, in vitro maturation and embryo transplantation.

 

   Purpose:

  • To reduce or prevent the potential for transmission of pathogens from a mouse that is being placed in inside a barrier housing facility.

 

Procedure:

For heterozygous lines:

  • Investigator will provide 3-5 males (8-20 weeks old)
  • Rederivation Core will purchase wild type females (up to 10) from the appropriate background (from a preferred vendor)

For homozygous lines:

  • Investigator will provide 5-10 males (8-20 weeks old)
  • 10 homozygous females (3 to 6 weeks old)
  • Donor females are superovulated and mated naturally to stub males.
  • Embryos are collected, pooled, and washed to reduce the risk of pathogen transfer.
  • The embryos are surgically transferred, using aseptic technique, into reproductive tract of pseudopregnant recipient females.
  • If other recipients are requested, the procedure falls under customized rederivation services.

 

Steps Involved:

  1. Rederivation request form - now through RIO.
  2. Provide documentation where the mice are coming from (defined, conventional, undefined thus indicating that additional safeguards for pathogen removal are necessary).
  3. Provide males and females of the appropriate age
Note: Please inform core of genotyping final results and when to sacrifice the original mice.

 

The Rederivation Core will:

  • Return to investigator at least one rederived litter or a minimum of one breeding pair (SPF health status).
  • Provide accurate documentation of matings upon request.
  • Rederivation core does not guarantee success.

 

All movement of mice must be coordinated by the first contacting the rederivation core and then through the LARC business office as an inter-facility transport. 

 

Procedure rates:

Please note: Rederivation Rates have increased effective 7/8/24. These rates are still below industry market value.

  • Cost of rederivation is $1,240.
  • Cost of recipients is $443 per strain rederived.
  • Cost of egg doners (i.e. any wild type females purchased) and per diem will be recharged to the PI's account.
  • The PI has the option to cryopreserve sperm from 2 males used for rederivation at an additional cost (Click here for Cryo Core Link).
  • If more than 2 rederivation procedures are needed, it will fall under the category of customized rederivation and extra charges may be incurred.

 

 

 

 

Time line:

Approximately 12-16 week

Back to Top